Online Pharmacy US » Human http://medsnetic.com/blog Viagra, Xanax, Cialis, Phentermine, Levitra Fri, 10 Sep 2010 21:39:18 +0000 http://wordpress.org/?v=2.9.2 en hourly 1 Caffeine augments Alprazolam induced cytotoxicity in human cell lines. http://medsnetic.com/blog/2010/05/30/caffeine-augments-alprazolam-induced-cytotoxicity-in-human-cell-lines-2/ http://medsnetic.com/blog/2010/05/30/caffeine-augments-alprazolam-induced-cytotoxicity-in-human-cell-lines-2/#comments Sun, 30 May 2010 13:45:02 +0000 drugs http://medsnetic.com/blog/2010/05/30/caffeine-augments-alprazolam-induced-cytotoxicity-in-human-cell-lines-2/ Authors: Saha B, Mukherjee A, Samanta S, Saha P, Ghosh AK, Santra CR, Karmakar P
Combined effects of alprazolam (Alp), a member of benzodiazepine group of drugs and caffeine on human cell lines, HeLa and THP1 were investigated in this stu dy. Alp mediated cytotoxicity was enhanced while caffeine was present. The cell death was confirmed by observing morphological changes, LDH assay and membrane anisotropic study. Also such combined effects induced elevated level of ROS and depletion of GSH. The mechanism of cell death induced by simultaneous treatment of Alp and caffeine was associated with the calcium-mediated activation of mu-calpain, release of lysosomal protease cathepsin B, activation of PARP and cleavage of caspase 3. Our results indicate that, Alp alon e induces apoptosis in human…

]]> http://medsnetic.com/blog/2010/05/30/caffeine-augments-alprazolam-induced-cytotoxicity-in-human-cell-lines-2/feed/ 0 Modulation of human risky decision making by flunitrazepam http://medsnetic.com/blog/2010/05/29/modulation-of-human-risky-decision-making-by-flunitrazepam-2/ http://medsnetic.com/blog/2010/05/29/modulation-of-human-risky-decision-making-by-flunitrazepam-2/#comments Sat, 29 May 2010 20:25:18 +0000 drugs http://medsnetic.com/blog/2010/05/29/modulation-of-human-risky-decision-making-by-flunitrazepam-2/ Conclusions  At sufficiently high doses, flunitrazepam can engender increases in risky decision making. Globally, these changes appear
similar to previous effects we have observed after acute administration of alcohol and alprazolam. As suggested by the EVM
outcomes, the mechanisms underlying the changes in risky decision making are more similar to alprazolam than alcohol.

Content Type Journal ArticleCategory Original InvestigationDOI 10.1007/s00213-007-0951-4Authors
Scott D. Lane, University of Texas Health Science Center–Houston Psychiatry and Behavioral Sciences 1300 Moursu nd Street Houston TX 77 030 USADon R. Cherek, University of Texas Health Science Center–Houston Psychiatry and Behavioral Sciences 1300 Moursund Street Houston TX 77030 USASylvain O. Nouvion, U…

]]> http://medsnetic.com/blog/2010/05/29/modulation-of-human-risky-decision-making-by-flunitrazepam-2/feed/ 0 Erratum to: Validating a human model for anxiety using startle potentiated by cue and context: the effects of alprazolam, pregabalin, and diphenhydramine http://medsnetic.com/blog/2010/05/29/erratum-to-validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine-2/ http://medsnetic.com/blog/2010/05/29/erratum-to-validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine-2/#comments Sat, 29 May 2010 13:10:11 +0000 drugs http://medsnetic.com/blog/2010/05/29/erratum-to-validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine-2/ Content Type Journal ArticleCategory ErratumDOI 10.1007/s00213-010-1837-4Authors
J. M. P. Baas, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Heidelberglaan 2 3584 CS Ut recht The NetherlandsN. Mol, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsJ. L. Kenemans, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsE. P. Prinssen, CNS Research, F. Hoffmann-La Roche, Clinical Development Basel SwitzerlandI. Niklson, CNS Research, F. Hoffmann-La Roche, Clinical Development Base l SwitzerlandC. Xia-Chen, Centre for Human Drug Resear…

]]> http://medsnetic.com/blog/2010/05/29/erratum-to-validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine-2/feed/ 0 Discovery That Viagra Enhances Fetal Growth In Female Sheep May Have Human Implications http://medsnetic.com/blog/2010/05/28/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-4/ http://medsnetic.com/blog/2010/05/28/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-4/#comments Fri, 28 May 2010 18:59:34 +0000 drugs http://medsnetic.com/blog/2010/05/28/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-4/ A joke among two Texas AgriLife Research scientists later turned into a fully-funded study. Viagra can aid fetal development in female sheep. Female sheep (ewes) are an agriculturally important species, which can se rve as an excellent animal model for studying the physiology of human pregnancy, the researchers said… (Source: Biology / Biochemistry News From Medical News Today)

]]> http://medsnetic.com/blog/2010/05/28/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-4/feed/ 0 Validated LC-MS/MS assay for the quantitative determination of vardenafil in human plasma and its application to a pharmacokinetic study. http://medsnetic.com/blog/2010/05/26/validated-lc-msms-assay-for-the-quantitative-determination-of-vardenafil-in-human-plasma-and-its-application-to-a-pharmacokinetic-study-2/ http://medsnetic.com/blog/2010/05/26/validated-lc-msms-assay-for-the-quantitative-determination-of-vardenafil-in-human-plasma-and-its-application-to-a-pharmacokinetic-study-2/#comments Wed, 26 May 2010 01:32:59 +0000 drugs http://medsnetic.com/blog/2010/05/26/validated-lc-msms-assay-for-the-quantitative-determination-of-vardenafil-in-human-plasma-and-its-application-to-a-pharmacokinetic-study-2/ Authors: Lake ST, Altman PM, Vaisman J, Addison RS
A sensitive high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) assay has been developed for the quantitative analysis of vardenafil in human plasma. Vardenafil and the internal standard, alprazolam, were extracted from 0.2 mL aliquots of alkalinized plasma by a single solvent extractio n into hexane : dichloromethane. Reversed-phase chromatographic separation was affected by gradient elution with mobile phases consisting of 10 mM ammonium formate pH 7.0 (solvent A) and methanol (100%, solvent B), delivered at a flow rat e of 0.4 mL/min. The analytes were detected by using an electrospray ion source on a 4000 QTrap triple quadrupole mass spectrometer operating in positive ionization mode. The mass transitions we…

]]> http://medsnetic.com/blog/2010/05/26/validated-lc-msms-assay-for-the-quantitative-determination-of-vardenafil-in-human-plasma-and-its-application-to-a-pharmacokinetic-study-2/feed/ 0 Experimental design for optimization of microwave-assisted extraction of benzodiazepines in human plasma. http://medsnetic.com/blog/2010/05/25/experimental-design-for-optimization-of-microwave-assisted-extraction-of-benzodiazepines-in-human-plasma-2/ http://medsnetic.com/blog/2010/05/25/experimental-design-for-optimization-of-microwave-assisted-extraction-of-benzodiazepines-in-human-plasma-2/#comments Tue, 25 May 2010 13:56:58 +0000 drugs http://medsnetic.com/blog/2010/05/25/experimental-design-for-optimization-of-microwave-assisted-extraction-of-benzodiazepines-in-human-plasma-2/ Authors: Fernández P, Vázquez C, Lorenzo RA, Carro AM, Alvarez I, Cabarcos P
A simple and fast microwave-assisted-extraction (MAE) method has been evaluated as an alternative to solid-phase extraction (SPE) for the determination of six benzodiazepines widely prescribed in European countries (alprazolam, bromazepam, diazepam, lorazepam, lormetazepam and tetrazepam) in human plasma. For MAE optimization a Doehlert experimental design was used with extraction time, temperature and solvent volume as influential parameters. A desirability function was employed in addition to the simultaneous optimization of the MAE condition s. The analysis of variance showed that the solvent volume had a positive influence on the extraction of all the analytes tested, achieving a statistically sig…

]]> http://medsnetic.com/blog/2010/05/25/experimental-design-for-optimization-of-microwave-assisted-extraction-of-benzodiazepines-in-human-plasma-2/feed/ 0 Discovery That Viagra Enhances Fetal Growth In Female Sheep May Have Human Implications http://medsnetic.com/blog/2010/05/22/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-3/ http://medsnetic.com/blog/2010/05/22/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-3/#comments Sat, 22 May 2010 21:00:32 +0000 drugs http://medsnetic.com/blog/2010/05/22/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-3/ A joke among two Texas AgriLife Research scientists later turned into a fully-funded study. Viagra can aid fetal development in female sheep. Female sheep (ewes) are an agriculturally important species, which can serve as an excellent animal model for studying the physiology of human pregnancy, the researchers said. Viagr a (sildenafil citrate), which is used to treat male erectile dysfunction, enhanced blood flow in pregnant female sheep, helping send vital amino acids and other nutrients needed in fetal development… (Source: Health News from Medical News Today)

]]> http://medsnetic.com/blog/2010/05/22/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-3/feed/ 0 Validating a human model for anxiety using startle potentiated by cue and context: the effects of alprazolam, pregabalin, and diphenhydramine http://medsnetic.com/blog/2010/05/22/validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine-2/ http://medsnetic.com/blog/2010/05/22/validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine-2/#comments Sat, 22 May 2010 20:05:08 +0000 drugs http://medsnetic.com/blog/2010/05/22/validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine-2/ Conclusion  Even though fear-potentiated startle may be used to translate preclini cal evidence to human populations, methodological issues
still hamper the application of this model to early screening of putative anxiolytic drugs.

Content Type Journal ArticleCategory Original InvestigationDOI 10.1007/s00213-009-1516-5Authors
J. M. P. Baas, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsN. Mol, Utrecht Univer sity Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsJ. L. Kenemans, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Hei…

]]> http://medsnetic.com/blog/2010/05/22/validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine-2/feed/ 0 Simultaneous determination of benzodiazepines and their metabolites in human serum by liquid chromatography-tandem mass spectrometry using a high-resolution octadecyl silica column compatible with aqueous compounds. http://medsnetic.com/blog/2010/05/21/simultaneous-determination-of-benzodiazepines-and-their-metabolites-in-human-serum-by-liquid-chromatography-tandem-mass-spectrometry-using-a-high-resolution-octadecyl-silica-column-compatible-with-a-2/ http://medsnetic.com/blog/2010/05/21/simultaneous-determination-of-benzodiazepines-and-their-metabolites-in-human-serum-by-liquid-chromatography-tandem-mass-spectrometry-using-a-high-resolution-octadecyl-silica-column-compatible-with-a-2/#comments Fri, 21 May 2010 23:32:01 +0000 drugs http://medsnetic.com/blog/2010/05/21/simultaneous-determination-of-benzodiazepines-and-their-metabolites-in-human-serum-by-liquid-chromatography-tandem-mass-spectrometry-using-a-high-resolution-octadecyl-silica-column-compatible-with-a-2/ Authors: Nakamura M, Ohmori T, Itoh Y, Terashita M, Hirano K
A liquid chromatography-tandem ma ss spectrometry (LC-MS/MS) method using a high-resolution octa decyl silica column compatible with aqueous compounds was developed for the simultaneous determination of benzodiazepines and their metabolites in human serum. This method enabled us to determine multiple benzodiazepines, including flurazepam, bromazepam, chlordiazepoxide, nitrazepam, clonazepam, flunitrazepam, estazolam, clobazam, lorazepam, alprazolam, triazolam, brotizolam, fludiazepam, diazepam, quazepam, prazepam and their metabolites such as 7-aminonitrazepam, 7-aminoclonazepam, 7-acetamidonitrazepam, N-desmethylclobazam and N-desmethyldiazepam. The analytes spiked into human serum were subjected to solid-phase extraction foll…

]]> http://medsnetic.com/blog/2010/05/21/simultaneous-determination-of-benzodiazepines-and-their-metabolites-in-human-serum-by-liquid-chromatography-tandem-mass-spectrometry-using-a-high-resolution-octadecyl-silica-column-compatible-with-a-2/feed/ 0 A comparison of the effects of a subtype selective and non-selective benzodiazepine receptor agonist in two CO2 models of experimental human anxiety http://medsnetic.com/blog/2010/05/21/a-comparison-of-the-effects-of-a-subtype-selective-and-non-selective-benzodiazepine-receptor-agonist-in-two-co2-models-of-experimental-human-anxiety-2/ http://medsnetic.com/blog/2010/05/21/a-comparison-of-the-effects-of-a-subtype-selective-and-non-selective-benzodiazepine-receptor-agonist-in-two-co2-models-of-experimental-human-anxiety-2/#comments Fri, 21 May 2010 14:51:22 +0000 drugs http://medsnetic.com/blog/2010/05/21/a-comparison-of-the-effects-of-a-subtype-selective-and-non-selective-benzodiazepine-receptor-agonist-in-two-co2-models-of-experimental-human-anxiety-2/ In conclusion, our results show that zolpidem shows some anxiolytic efficacy in the 7.5% CO2 model, similar to alprazolam, and this is the first report of such an effect of zolpidem in a model of a nxiety. These and other studies of benzodiazepines in clinical and volunteer studies suggest a definite role of the GABA-A receptor in CO2-induced anxiety, and it would be of interest to examine other GABA-A receptor subtype selective drugs, which are now in early phase clinical studies and are showing selective efficacy in pharmacodynamic studies. (Source: Journal of Psychopharmacology)

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]]> http://medsnetic.com/blog/2010/05/21/a-comparison-of-the-effects-of-a-subtype-selective-and-non-selective-benzodiazepine-receptor-agonist-in-two-co2-models-of-experimental-human-anxiety-2/feed/ 0 Benzodiazepines medazepam and midazolam are activators of pregnane X receptor and weak inducers of CYP3A4: Investigation in primary cultures of human hepatocytes and hepatocarcinoma cell lines. http://medsnetic.com/blog/2010/05/21/benzodiazepines-medazepam-and-midazolam-are-activators-of-pregnane-x-receptor-and-weak-inducers-of-cyp3a4-investigation-in-primary-cultures-of-human-hepatocytes-and-hepatocarcinoma-cell-lines-2/ http://medsnetic.com/blog/2010/05/21/benzodiazepines-medazepam-and-midazolam-are-activators-of-pregnane-x-receptor-and-weak-inducers-of-cyp3a4-investigation-in-primary-cultures-of-human-hepatocytes-and-hepatocarcinoma-cell-lines-2/#comments Fri, 21 May 2010 12:47:37 +0000 drugs http://medsnetic.com/blog/2010/05/21/benzodiazepines-medazepam-and-midazolam-are-activators-of-pregnane-x-receptor-and-weak-inducers-of-cyp3a4-investigation-in-primary-cultures-of-human-hepatocytes-and-hepatocarcinoma-cell-lines-2/ In conclusion, Alprazolam, Bromazepam, Chlordiazepoxide, Clonazepam, Di azepam, Lorazepam, Nitrazepam, Oxazepam, Tetrazepam and Triazolam can be considered as safe drugs in term of their inability to induce PXR- and AhR-dependent cytochrome P450 enzymes CYP1A2 and CYP3A4. Medazepam and Midazolam slightly activated pregnane X receptor and displayed weak potency to induce CYP3A4 mRNA in human hepatocytes.
PMID: 20080160 [PubMed - as supplied by publisher] (Source: Toxicology Letters)

]]> http://medsnetic.com/blog/2010/05/21/benzodiazepines-medazepam-and-midazolam-are-activators-of-pregnane-x-receptor-and-weak-inducers-of-cyp3a4-investigation-in-primary-cultures-of-human-hepatocytes-and-hepatocarcinoma-cell-lines-2/feed/ 2 Erratum to: Validating a human model for anxiety using startle potentiated by cue and context: the effects of alprazolam, pregabalin, and diphenhydramine http://medsnetic.com/blog/2010/04/26/erratum-to-validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine/ http://medsnetic.com/blog/2010/04/26/erratum-to-validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine/#comments Mon, 26 Apr 2010 13:44:17 +0000 drugs http://medsnetic.com/blog/2010/04/26/erratum-to-validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine/ Content Type Journal ArticleCategory ErratumDOI 10.1007/s00213-010-1837-4Authors
J. M. P. Baas, Utrecht University Department of Experimental Psychology, Faculty of Social S ciences Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsN. Mol, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsJ. L. Kenemans, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsE. P. Prinssen, CNS Research, F. Hoffmann-La Roche, Clinical Development Basel SwitzerlandI. Niklson, CNS Research, F. Hoffmann-La Roch e, Clinical Development Basel SwitzerlandC. Xia-Chen, Centre for Human Drug Resear…

]]> http://medsnetic.com/blog/2010/04/26/erratum-to-validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine/feed/ 0 Experimental design for optimization of microwave-assisted extraction of benzodiazepines in human plasma. http://medsnetic.com/blog/2010/03/08/experimental-design-for-optimization-of-microwave-assisted-extraction-of-benzodiazepines-in-human-plasma/ http://medsnetic.com/blog/2010/03/08/experimental-design-for-optimization-of-microwave-assisted-extraction-of-benzodiazepines-in-human-plasma/#comments Mon, 08 Mar 2010 07:18:20 +0000 drugs http://medsnetic.com/blog/2010/03/08/experimental-design-for-optimization-of-microwave-assisted-extraction-of-benzodiazepines-in-human-plasma/ Authors: Fernández P, Vázquez C, Lorenzo RA, Carro AM, Alvarez I, Cabarcos P
A simple and fast microwave-assisted-extraction (MAE) method has been evaluated as an alternative to solid-phase e xtraction (SPE) for the determination of six benzodiazepines widely prescribed in European countries (alprazolam, bromazepam, diazepam, lorazepam, lormetazepam and tetrazepam) in human plasma. For MAE optimization a Doehlert experimental design was used with extraction time, temperature and solvent volume as influential parameters. A desirability function was employed in addition to the simultaneous optimization of the MAE conditions. The analysis of variance showed that the solvent volume had a positive influence on the extraction of all the analytes tested, achieving a statistically sig…

]]> http://medsnetic.com/blog/2010/03/08/experimental-design-for-optimization-of-microwave-assisted-extraction-of-benzodiazepines-in-human-plasma/feed/ 0 Discovery That Viagra Enhances Fetal Growth In Female Sheep May Have Human Implications http://medsnetic.com/blog/2010/03/04/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-2/ http://medsnetic.com/blog/2010/03/04/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-2/#comments Thu, 04 Mar 2010 09:57:13 +0000 drugs http://medsnetic.com/blog/2010/03/04/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-2/ A joke among two Texas AgriLife Research scientists later turned into a fully-funded study. Viagra can aid fetal development in female sheep. Female sheep (ewes) are an agriculturally important species, which can serve as an excellent animal model for studying the physiology of human pregnancy, the researchers said… (Source: Biology / Biochemistry News From Medical N ews Today)

]]> http://medsnetic.com/blog/2010/03/04/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications-2/feed/ 0 Benzodiazepines medazepam and midazolam are activators of pregnane X receptor and weak inducers of CYP3A4: Investigation in primary cultures of human hepatocytes and hepatocarcinoma cell lines. http://medsnetic.com/blog/2010/03/01/benzodiazepines-medazepam-and-midazolam-are-activators-of-pregnane-x-receptor-and-weak-inducers-of-cyp3a4-investigation-in-primary-cultures-of-human-hepatocytes-and-hepatocarcinoma-cell-lines/ http://medsnetic.com/blog/2010/03/01/benzodiazepines-medazepam-and-midazolam-are-activators-of-pregnane-x-receptor-and-weak-inducers-of-cyp3a4-investigation-in-primary-cultures-of-human-hepatocytes-and-hepatocarcinoma-cell-lines/#comments Mon, 01 Mar 2010 04:35:25 +0000 drugs http://medsnetic.com/blog/2010/03/01/benzodiazepines-medazepam-and-midazolam-are-activators-of-pregnane-x-receptor-and-weak-inducers-of-cyp3a4-investigation-in-primary-cultures-of-human-hepatocytes-and-hepatocarcinoma-cell-lines/ In conclusion, Alprazolam, Bromazepam, Chlordiazepoxide, Clonazepam, Diazepam, Lorazepam, Nitrazepam, Oxazepam, Tetrazepam and Triazolam can be considered as safe drugs in term of their inability to induce PXR- and AhR-dependent cytochrome P450 enzymes CYP1A2 and CYP3A4. Medazepam and Midazolam slightly activated pregnane X receptor and displayed weak potency to induce CYP3A4 mRNA in human hepatocytes.
PMI D: 20080160 [PubMed - as supplied by publisher] (Source: Toxicology Letters)

]]> http://medsnetic.com/blog/2010/03/01/benzodiazepines-medazepam-and-midazolam-are-activators-of-pregnane-x-receptor-and-weak-inducers-of-cyp3a4-investigation-in-primary-cultures-of-human-hepatocytes-and-hepatocarcinoma-cell-lines/feed/ 0 Validating a human model for anxiety using startle potentiated by cue and context: the effects of alprazolam, pregabalin, and diphenhydramine http://medsnetic.com/blog/2010/02/17/validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine/ http://medsnetic.com/blog/2010/02/17/validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine/#comments Wed, 17 Feb 2010 17:49:05 +0000 drugs http://medsnetic.com/blog/2010/02/17/validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine/ Conclusion  Even though fear-potentiated startle may be used to translate preclinical evidence to human populations, methodological issues
still hamper the application of this model to early screening of putative anxiolytic drugs.

Content Type Journal ArticleCategory Original InvestigationDOI 10.1007/s00213-009-1516-5Authors
J. M. P. Baas, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsN. Mol, Utrecht Univ ersity Department of Experimental Psychology, Faculty of Social Scien ces Van Unnik Building, Heidelberglaan 2 3584 CS Utrecht The NetherlandsJ. L. Kenemans, Utrecht University Department of Experimental Psychology, Faculty of Social Sciences Van Unnik Building, Hei…

]]> http://medsnetic.com/blog/2010/02/17/validating-a-human-model-for-anxiety-using-startle-potentiated-by-cue-and-context-the-effects-of-alprazolam-pregabalin-and-diphenhydramine/feed/ 0 A comparison of the effects of a subtype selective and non-selective benzodiazepine receptor agonist in two CO2 models of experimental human anxiety http://medsnetic.com/blog/2010/02/16/a-comparison-of-the-effects-of-a-subtype-selective-and-non-selective-benzodiazepine-receptor-agonist-in-two-co2-models-of-experimental-human-anxiety/ http://medsnetic.com/blog/2010/02/16/a-comparison-of-the-effects-of-a-subtype-selective-and-non-selective-benzodiazepine-receptor-agonist-in-two-co2-models-of-experimental-human-anxiety/#comments Tue, 16 Feb 2010 08:55:34 +0000 drugs http://medsnetic.com/blog/2010/02/16/a-comparison-of-the-effects-of-a-subtype-selective-and-non-selective-benzodiazepine-receptor-agonist-in-two-co2-models-of-experimental-human-anxiety/ In conclusion, our results show that zolpidem shows some anxiolytic efficacy in the 7.5% CO2 model, similar to alprazolam, and this is the first report of such an effect of zolpidem in a model of anxiety. These and other studies of benzodiazepines in clinical and volunteer studies suggest a definite role of the GABA-A rec eptor in CO2-induced anxiety, and it would be of interest to examine other GABA-A receptor subtype selective drugs, which are now in early phase clinical studies and are showing selective efficacy in pharmacodynamic studies. (Source: Journal of Psychopharmacology)

]]> http://medsnetic.com/blog/2010/02/16/a-comparison-of-the-effects-of-a-subtype-selective-and-non-selective-benzodiazepine-receptor-agonist-in-two-co2-models-of-experimental-human-anxiety/feed/ 0 Validated LC-MS/MS assay for the quantitative determination of vardenafil in human plasma and its application to a pharmacokinetic study. http://medsnetic.com/blog/2010/02/16/validated-lc-msms-assay-for-the-quantitative-determination-of-vardenafil-in-human-plasma-and-its-application-to-a-pharmacokinetic-study/ http://medsnetic.com/blog/2010/02/16/validated-lc-msms-assay-for-the-quantitative-determination-of-vardenafil-in-human-plasma-and-its-application-to-a-pharmacokinetic-study/#comments Tue, 16 Feb 2010 02:07:18 +0000 drugs http://medsnetic.com/blog/2010/02/16/validated-lc-msms-assay-for-the-quantitative-determination-of-vardenafil-in-human-plasma-and-its-application-to-a-pharmacokinetic-study/ Authors: Lake ST, Altman PM, Vaisman J, Addison RS
A sensitive high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) assay has been developed for the quantitative analysis of vardenafil in hum an plasma. Vardenafil and the internal standard, alprazolam, were extracted from 0.2 mL aliquots of alkalinized plasma by a single solvent extraction into hexane : dichloromethane. Reversed-phase chromatographic separation was affected by gradient elution with mobile phases consisting of 10 mM ammonium formate pH 7.0 (solvent A) and methanol (100%, solvent B), delivered at a flow rate of 0.4 mL/min. The analytes were detected by using an electrospray ion source on a 4000 QTrap triple quadrupole mass spectrometer operating in positive ionization mode. The mass transitions we…

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]]> http://medsnetic.com/blog/2010/02/16/validated-lc-msms-assay-for-the-quantitative-determination-of-vardenafil-in-human-plasma-and-its-application-to-a-pharmacokinetic-study/feed/ 0 Modulation of human risky decision making by flunitrazepam http://medsnetic.com/blog/2010/02/15/modulation-of-human-risky-decision-making-by-flunitrazepam/ http://medsnetic.com/blog/2010/02/15/modulation-of-human-risky-decision-making-by-flunitrazepam/#comments Mon, 15 Feb 2010 11:17:38 +0000 drugs http://medsnetic.com/blog/2010/02/15/modulation-of-human-risky-decision-making-by-flunitrazepam/ C onclusions  At sufficiently high do ses, flunitrazepam can engender increases in risky decision making. Globally, these changes appear
similar to previous effects we have observed after acute administration of alcohol and alprazolam. As suggested by the EVM
outcomes, the mechanisms underlying the changes in risky decision making are more similar to alprazolam than alcohol.

Content Type Journal ArticleCategory Original InvestigationDOI 10.1007/s00213-007-0951-4Authors
Scott D. Lane, University of Texas Health Science Center–Houston Psychiatry and Behavioral Sciences 1300 Moursund Street Houston TX 77030 USADon R. Cherek, University of Texas Health Science Center–Houston Psychiatry and Behavioral Sciences 1300 Moursund Street Houston TX 77030 USASylvain O. Nouvion, U…

]]> http://medsnetic.com/blog/2010/02/15/modulation-of-human-risky-decision-making-by-flunitrazepam/feed/ 0 Limitations to animal data on sildenafil citrate (Viagra) in the human risk assessment http://medsnetic.com/blog/2010/02/11/limitations-to-animal-data-on-sildenafil-citrate-viagra-in-the-human-risk-assessment/ http://medsnetic.com/blog/2010/02/11/limitations-to-animal-data-on-sildenafil-citrate-viagra-in-the-human-risk-assessment/#comments Thu, 11 Feb 2010 02:06:13 +0000 drugs http://medsnetic.com/blog/2010/02/11/limitations-to-animal-data-on-sildenafil-citrate-viagra-in-the-human-risk-assessment/ To the Editor: The article titled Sildenafil citrate (Viagra) impairs fertilization and early embryo development in mice by Glenn et al. was of great interest. However, the presented data should be clarified from a teratological point of view, as it is insufficient for any further toxicological evaluation, in particular the human risk assessment. Without recording the initial number of females selected to each examined group, it is impossible to calculate the mating and fertility indexes that are crucial for s tage A of developmental toxicological studies . In addition, the postimplantation loss on day 4 should be calculated. (Source: Fertility and Sterility)

]]> http://medsnetic.com/blog/2010/02/11/limitations-to-animal-data-on-sildenafil-citrate-viagra-in-the-human-risk-assessment/feed/ 0 Simultaneous determination of benzodiazepines and their metabolites in human serum by liquid chromatography-tandem mass spectrometry using a high-resolution octadecyl silica column compatible with aqueous compounds. http://medsnetic.com/blog/2010/02/09/simultaneous-determination-of-benzodiazepines-and-their-metabolites-in-human-serum-by-liquid-chromatography-tandem-mass-spectrometry-using-a-high-resolution-octadecyl-silica-column-compatible-with-aqu/ http://medsnetic.com/blog/2010/02/09/simultaneous-determination-of-benzodiazepines-and-their-metabolites-in-human-serum-by-liquid-chromatography-tandem-mass-spectrometry-using-a-high-resolution-octadecyl-silica-column-compatible-with-aqu/#comments Tue, 09 Feb 2010 19:13:30 +0000 drugs http://medsnetic.com/blog/2010/02/09/simultaneous-determination-of-benzodiazepines-and-their-metabolites-in-human-serum-by-liquid-chromatography-tandem-mass-spectrometry-using-a-high-resolution-octadecyl-silica-column-compatible-with-aqu/ Authors: Nakamura M, Ohmori T Itoh Y, Terashita M, Hirano K
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using, a high-resolution octadecyl silica column compatible with aqueous compounds was developed for the simultaneous determination of benzodiazepines and their metabolites in human serum. This method enabled us to determine multiple benzodiazepines, including flurazepam, bromazepam, chlordiazepoxide, nitrazepam, clonazepam, flunitrazepam, estazolam, clobazam, lorazepam, alprazolam, triazolam, brotizolam, fludiazepam, diazepam, quazepam, prazepam and their metab olites such as 7-aminonitrazepam, 7-aminoclonazepam, 7-acetamidonitrazepam, N-desmethylclobazam and N-desmethyldiazepam. The analytes spiked into human serum were subjected to solid-phase extraction foll.

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]]> http://medsnetic.com/blog/2010/02/09/simultaneous-determination-of-benzodiazepines-and-their-metabolites-in-human-serum-by-liquid-chromatography-tandem-mass-spectrometry-using-a-high-resolution-octadecyl-silica-column-compatible-with-aqu/feed/ 0 Discovery That Viagra Enhances Fetal Growth In Female Sheep May Have Human Implications http://medsnetic.com/blog/2010/02/06/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications/ http://medsnetic.com/blog/2010/02/06/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications/#comments Sat, 06 Feb 2010 20:28:12 +0000 drugs http://medsnetic.com/blog/2010/02/06/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications/ A joke among two Texas AgriLife Research scientists later turned into a fully-funded study. Viagra can aid fetal development in female sheep. Female sheep (ewes) are an agriculturally important species, which can serve as an excellent animal model for studying the physiology of human pregnancy, the researchers said. Viagra (sildenafil citrate), which is used to treat male erectile dysfunction, enhanced blood flow in pregnant fema le sheep, helping send vital amino acids and other nutrients needed in fetal development… (Source: Health News from Medical News Today)

]]> http://medsnetic.com/blog/2010/02/06/discovery-that-viagra-enhances-fetal-growth-in-female-sheep-may-have-human-implications/feed/ 0 Caffeine augments Alprazolam induced cytotoxicity in human cell lines. http://medsnetic.com/blog/2010/02/06/caffeine-augments-alprazolam-induced-cytotoxicity-in-human-cell-lines/ http://medsnetic.com/blog/2010/02/06/caffeine-augments-alprazolam-induced-cytotoxicity-in-human-cell-lines/#comments Sat, 06 Feb 2010 12:50:44 +0000 drugs http://medsnetic.com/blog/2010/02/06/caffeine-augments-alprazolam-induced-cytotoxicity-in-human-cell-lines/ Auth ors: Saha B, Mukherjee A, Samanta S, Saha P, Ghosh AK, Santra CR, Karmakar P
Combined effects of alprazolam (Alp), a member of benzodiazepine group of drugs and caffeine on human cell lines, HeLa and THP1 were investigated in this study. Alp mediated cytotoxicity was enhanced while caffeine was present. The cell death was confirmed by observing morphological changes, LDH assay and membrane anisotropic study. Also such combined effects induced elevated level of ROS and depletion of GSH. The mechanism of cell death induced by simultaneous treatment of Alp and caffeine was associat ed with the calcium-mediated activation of mu-calpain, release of lysosomal protease cathepsin B, activation of PARP and cleavage of caspase 3. Our results indicate that, Alp alone induces apoptosis in human…

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]]> http://medsnetic.com/blog/2010/02/06/caffeine-augments-alprazolam-induced-cytotoxicity-in-human-cell-lines/feed/ 0